Thursday, January 21, 2016

Candy Electrophoresis Lab Analysis

All the dyes that were found matched up with  reference dyes in color, size, and area in the gel except for the green dye, which split in 2 and had a blue band and a yellow band. This is probably because the color green is created from a combination of those colors. and the mixture was split during the electrophoresis. 

The citrus red 2 dye is most similar to the dyes in the lab, specifically red 40, because it has the relatively same structure and size un like the other dyes, which are oddly shaped. Due to the similarities between these dyes, they would probably move at the same rate and to the same area in the gel.

Dog food companies probably put different color dyes in their foods because it might make the food seem more appetizing and the color may attract dogs towards it. Also, the color may give it the artificial look of meat and can make it look better compared to an all natural look.

The reason people might prefer artificial dyes over natural dyes is because artificial dyes can come in a larger variety and are cheeper to create and process, while natural dyes must be obtained from a natural source and may not have the desired quality or color.

The forces that control the distance tin which the dyes migrate are the size of the pieces of DNA along with the structure of the dye. These two control the distance because if the piece is smaller, then it travels farther and the structure may cause it to be larger or smaller.

The force of the electricity running through the gel moves the dye, which moves them from a negatively charged side to a positively charged size.

The movement caused by the charge distribution allows the DNA to move in little holes in the gel, thus splitting them by size since the smaller particles take less time to move than the larger ones. 

The DNA molecules with those weights will separate through the holes, but some will just take longer to migrate. In order of farthest to closest from the starting point it would be: 600, 1000, 2000, with 5000 lagging far behind.

No comments:

Post a Comment